Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 954218..954912 Replicon chromosome
Accession NZ_CP042846
Organism Escherichia coli strain JME64

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FVE20_RS04475 Protein ID WP_001263489.1
Coordinates 954514..954912 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FVE20_RS04470 Protein ID WP_000554758.1
Coordinates 954218..954511 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE20_RS04450 949850..950347 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FVE20_RS04455 950571..952283 - 1713 Protein_847 flagellar biosynthesis protein FlhA -
FVE20_RS04460 952255..953040 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FVE20_RS04465 953111..954166 + 1056 WP_001226164.1 DNA polymerase IV -
FVE20_RS04470 954218..954511 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FVE20_RS04475 954514..954912 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FVE20_RS04480 954922..955374 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FVE20_RS04485 955692..955898 + 207 Protein_853 RtcB family protein -
FVE20_RS04490 955894..956415 + 522 Protein_854 peptide chain release factor H -
FVE20_RS04495 956472..957929 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FVE20_RS04500 958190..958648 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 959244..959324 + 81 NuclAT_12 - -
- 959244..959324 + 81 NuclAT_12 - -
- 959244..959324 + 81 NuclAT_12 - -
- 959244..959324 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T136205 WP_001263489.1 NZ_CP042846:954514-954912 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T136205 NZ_CP042846:954514-954912 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT136205 WP_000554758.1 NZ_CP042846:954218-954511 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT136205 NZ_CP042846:954218-954511 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References