Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2792811..2793313 Replicon chromosome
Accession NZ_CP042845
Organism Escherichia coli strain JME65

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FVE21_RS13775 Protein ID WP_000767829.1
Coordinates 2792811..2793065 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FVE21_RS13780 Protein ID WP_001259255.1
Coordinates 2793062..2793313 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE21_RS13745 2787826..2788053 - 228 WP_000234896.1 sulfurtransferase TusA -
FVE21_RS13750 2788067..2789125 - 1059 WP_000492339.1 transport protein YeeE -
FVE21_RS13755 2789304..2790662 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FVE21_RS13760 2790652..2790714 - 63 WP_010723108.1 membrane protein YoeI -
FVE21_RS13765 2790929..2791858 - 930 WP_000803351.1 LysR family transcriptional regulator -
FVE21_RS13770 2791904..2792728 - 825 WP_000754737.1 SDR family oxidoreductase -
FVE21_RS13775 2792811..2793065 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FVE21_RS13780 2793062..2793313 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FVE21_RS13785 2793596..2793646 + 51 WP_001364200.1 his operon leader peptide -
FVE21_RS13790 2793792..2794691 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FVE21_RS13795 2794697..2796001 + 1305 WP_000009594.1 histidinol dehydrogenase -
FVE21_RS13800 2795998..2797068 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FVE21_RS13805 2797068..2798135 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136187 WP_000767829.1 NZ_CP042845:c2793065-2792811 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136187 NZ_CP042845:c2793065-2792811 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136187 WP_001259255.1 NZ_CP042845:c2793313-2793062 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136187 NZ_CP042845:c2793313-2793062 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References