Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 959099..959793 Replicon chromosome
Accession NZ_CP042845
Organism Escherichia coli strain JME65

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FVE21_RS04500 Protein ID WP_001263489.1
Coordinates 959395..959793 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FVE21_RS04495 Protein ID WP_000554758.1
Coordinates 959099..959392 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE21_RS04475 954731..955228 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FVE21_RS04480 955452..957164 - 1713 Protein_852 flagellar biosynthesis protein FlhA -
FVE21_RS04485 957136..957921 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FVE21_RS04490 957992..959047 + 1056 WP_001226164.1 DNA polymerase IV -
FVE21_RS04495 959099..959392 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FVE21_RS04500 959395..959793 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FVE21_RS04505 959803..960255 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FVE21_RS04510 960573..960779 + 207 Protein_858 RtcB family protein -
FVE21_RS04515 960775..961296 + 522 Protein_859 peptide chain release factor H -
FVE21_RS04520 961353..962810 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FVE21_RS04525 963071..963529 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 964125..964205 + 81 NuclAT_12 - -
- 964125..964205 + 81 NuclAT_12 - -
- 964125..964205 + 81 NuclAT_12 - -
- 964125..964205 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T136162 WP_001263489.1 NZ_CP042845:959395-959793 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T136162 NZ_CP042845:959395-959793 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT136162 WP_000554758.1 NZ_CP042845:959099-959392 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT136162 NZ_CP042845:959099-959392 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References