136151

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3980712..3981511 Replicon chromosome
Accession NZ_CP042844
Organism Escherichia coli strain JME66

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FVE22_RS19335 Protein ID WP_000347273.1
Coordinates 3981047..3981511 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FVE22_RS19330 Protein ID WP_001307405.1
Coordinates 3980712..3981047 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE22_RS19315 3976497..3977267 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
FVE22_RS19320 3977283..3978617 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FVE22_RS19325 3978992..3980563 + 1572 WP_001273753.1 galactarate dehydratase -
FVE22_RS19330 3980712..3981047 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FVE22_RS19335 3981047..3981511 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FVE22_RS19340 3981566..3982375 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FVE22_RS19345 3982624..3983904 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FVE22_RS19350 3983927..3984400 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FVE22_RS19355 3984411..3984782 + 372 Protein_3733 PTS sugar transporter subunit IIC -
FVE22_RS19360 3984778..3985335 + 558 Protein_3734 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3971564..3992495 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T136151 WP_000347273.1 NZ_CP042844:3981047-3981511 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T136151 NZ_CP055259:658491-658805 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT136151 WP_001307405.1 NZ_CP042844:3980712-3981047 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT136151 NZ_CP042844:3980712-3981047 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References