Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2793043..2793545 Replicon chromosome
Accession NZ_CP042844
Organism Escherichia coli strain JME66

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FVE22_RS13780 Protein ID WP_000767829.1
Coordinates 2793043..2793297 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FVE22_RS13785 Protein ID WP_001259255.1
Coordinates 2793294..2793545 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE22_RS13750 2788058..2788285 - 228 WP_000234896.1 sulfurtransferase TusA -
FVE22_RS13755 2788299..2789357 - 1059 WP_000492339.1 transport protein YeeE -
FVE22_RS13760 2789536..2790894 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FVE22_RS13765 2790884..2790946 - 63 WP_010723108.1 membrane protein YoeI -
FVE22_RS13770 2791161..2792090 - 930 WP_000803351.1 LysR family transcriptional regulator -
FVE22_RS13775 2792136..2792960 - 825 WP_000754737.1 SDR family oxidoreductase -
FVE22_RS13780 2793043..2793297 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FVE22_RS13785 2793294..2793545 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FVE22_RS13790 2793828..2793878 + 51 WP_001364200.1 his operon leader peptide -
FVE22_RS13795 2794024..2794923 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FVE22_RS13800 2794929..2796233 + 1305 WP_000009594.1 histidinol dehydrogenase -
FVE22_RS13805 2796230..2797300 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FVE22_RS13810 2797300..2798367 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136144 WP_000767829.1 NZ_CP042844:c2793297-2793043 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136144 NZ_CP042844:c2793297-2793043 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136144 WP_001259255.1 NZ_CP042844:c2793545-2793294 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136144 NZ_CP042844:c2793545-2793294 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References