Detailed information of TA system TA136119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 954218..954912
• Replicon: chromosome
• Accession: NZ_CP042844
• Organism: Escherichia coli strain JME66

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FVE22_RS04475
• Protein ID: WP_001263489.1
• Coordinates: 954514..954912 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FVE22_RS04470
• Protein ID: WP_000554758.1
• Coordinates: 954218..954511 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE22_RS04450	949850..950347	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FVE22_RS04455	950571..952283	-	1713	Protein_847	flagellar biosynthesis protein FlhA	-
FVE22_RS04460	952255..953040	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FVE22_RS04465	953111..954166	+	1056	WP_001226164.1	DNA polymerase IV	-
FVE22_RS04470	954218..954511	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FVE22_RS04475	954514..954912	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FVE22_RS04480	954922..955374	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FVE22_RS04485	955692..955898	+	207	Protein_853	RtcB family protein	-
FVE22_RS04490	955894..956415	+	522	Protein_854	peptide chain release factor H	-
FVE22_RS04495	956472..957929	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FVE22_RS04500	958190..958648	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	959244..959324	+	81	NuclAT_12	-	-
-	959244..959324	+	81	NuclAT_12	-	-
-	959244..959324	+	81	NuclAT_12	-	-
-	959244..959324	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	923827..998458	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T136119 WP_001263489.1 NZ_CP042844:954514-954912 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T136119 NZ_CP042844:954514-954912 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT136119 WP_000554758.1 NZ_CP042844:954218-954511 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT136119 NZ_CP042844:954218-954511 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3