Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2797924..2798426 Replicon chromosome
Accession NZ_CP042843
Organism Escherichia coli strain JME67

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FVE23_RS13805 Protein ID WP_000767829.1
Coordinates 2797924..2798178 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FVE23_RS13810 Protein ID WP_001259255.1
Coordinates 2798175..2798426 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE23_RS13775 2792939..2793166 - 228 WP_000234896.1 sulfurtransferase TusA -
FVE23_RS13780 2793180..2794238 - 1059 WP_000492339.1 transport protein YeeE -
FVE23_RS13785 2794417..2795775 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FVE23_RS13790 2795765..2795827 - 63 WP_010723108.1 membrane protein YoeI -
FVE23_RS13795 2796042..2796971 - 930 WP_000803351.1 LysR family transcriptional regulator -
FVE23_RS13800 2797017..2797841 - 825 WP_000754737.1 SDR family oxidoreductase -
FVE23_RS13805 2797924..2798178 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FVE23_RS13810 2798175..2798426 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FVE23_RS13815 2798709..2798759 + 51 WP_001364200.1 his operon leader peptide -
FVE23_RS13820 2798905..2799804 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FVE23_RS13825 2799810..2801114 + 1305 WP_000009594.1 histidinol dehydrogenase -
FVE23_RS13830 2801111..2802181 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FVE23_RS13835 2802181..2803248 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136101 WP_000767829.1 NZ_CP042843:c2798178-2797924 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136101 NZ_CP042843:c2798178-2797924 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136101 WP_001259255.1 NZ_CP042843:c2798426-2798175 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136101 NZ_CP042843:c2798426-2798175 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References