Detailed information of TA system TA136032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2207901..2208488
• Replicon: chromosome
• Accession: NZ_CP042807
• Organism: Rhodanobacter glycinis strain T01E-68

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4VRU4
• Locus tag: CS053_RS09760
• Protein ID: WP_008214582.1
• Coordinates: 2208210..2208488 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CS053_RS09755
• Protein ID: WP_008214581.1
• Coordinates: 2207901..2208200 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS053_RS09730	2203245..2203859	+	615	WP_147627282.1	hypothetical protein	-
CS053_RS09735	2203936..2204343	+	408	WP_147627283.1	hypothetical protein	-
CS053_RS09740	2204515..2205408	+	894	WP_147627284.1	type II toxin-antitoxin system death-on-curing family toxin	-
CS053_RS09745	2205451..2206371	-	921	WP_147627285.1	LysR family transcriptional regulator	-
CS053_RS09750	2206683..2207684	+	1002	WP_147627286.1	MBL fold metallo-hydrolase	-
CS053_RS09755	2207901..2208200	-	300	WP_008214581.1	HigA family addiction module antidote protein	Antitoxin
CS053_RS09760	2208210..2208488	-	279	WP_008214582.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS053_RS09765	2208855..2210441	-	1587	WP_172624129.1	SDR family oxidoreductase	-
CS053_RS09770	2210815..2212020	-	1206	WP_147627287.1	acetyl-CoA C-acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-73)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10690.15 Da
• Isoelectric Point: 9.8233

>T136032 WP_008214582.1 NZ_CP042807:c2208488-2208210 [Rhodanobacter glycinis]
MVRSFRCKDTQALFEGGDPRRFRAIKDAAIRKLSQLDAAHTLEFLRSPPGNQLEALKHDRTGQYSIRINRQWRVCFVWTT
SGPESVEIVDYH

Nucleotide

• Length: 279 bp

>T136032 NZ_CP042807:c2208488-2208210 [Rhodanobacter glycinis]
ATGGTTCGGAGCTTCCGCTGCAAGGACACGCAGGCGTTGTTCGAAGGCGGTGACCCGCGACGGTTCCGCGCCATCAAGGA
TGCTGCCATACGCAAGCTGTCCCAACTCGATGCCGCGCACACGCTGGAGTTTCTGCGTTCGCCACCGGGCAACCAGCTCG
AAGCCCTCAAGCATGATCGCACTGGCCAGTACAGCATCCGCATCAATAGGCAATGGCGTGTGTGCTTTGTGTGGACGACC
AGTGGGCCGGAAAGCGTCGAAATCGTGGATTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11203.99 Da
• Isoelectric Point: 10.0844

>AT136032 WP_008214581.1 NZ_CP042807:c2208200-2207901 [Rhodanobacter glycinis]
MKLPVNRMRPVHPGEILREDYLVPLDMSAHALAVALSVPATRIHEIVNERRGISADTAERLARYFSGDALSWLNLQATYD
LKTLATRKEILRRVEPRAA

Nucleotide

• Length: 300 bp

>AT136032 NZ_CP042807:c2208200-2207901 [Rhodanobacter glycinis]
ATGAAACTCCCCGTCAACCGCATGCGTCCCGTGCATCCCGGCGAAATTCTGCGCGAGGATTACCTTGTGCCGCTCGACAT
GAGCGCCCATGCATTGGCCGTCGCCCTGAGCGTGCCAGCCACACGCATCCACGAAATCGTCAATGAGCGCCGCGGTATCA
GCGCCGACACTGCCGAGCGTCTGGCGCGCTACTTCAGCGGCGATGCACTGTCATGGCTGAACCTGCAGGCGACCTACGAT
CTCAAGACACTCGCCACGCGCAAGGAGATCCTGCGCCGGGTAGAGCCTCGCGCTGCCTGA

Structures

Toxin

• AlphaFold DB: I4VRU4