Detailed information of TA system TA135970

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 121180..121606
• Replicon: plasmid pLD91-1-146kb
• Accession: NZ_CP042586
• Organism: Escherichia coli strain LD91-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FQR64_RS24010
• Protein ID: WP_001312861.1
• Coordinates: 121180..121338 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	121382..121606 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQR64_RS23965	116226..116453	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FQR64_RS23970	116547..117233	-	687	WP_000332484.1	PAS domain-containing protein	-
FQR64_RS23975	117424..117807	-	384	WP_000124981.1	relaxosome protein TraM	-
FQR64_RS23980	118084..118731	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
FQR64_RS23985	119028..119849	-	822	WP_001234469.1	DUF945 domain-containing protein	-
FQR64_RS23990	119971..120258	-	288	WP_000107535.1	hypothetical protein	-
FQR64_RS23995	120283..120489	-	207	WP_000547939.1	hypothetical protein	-
FQR64_RS24010	121180..121338	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	121382..121606	-	225	NuclAT_0	-	Antitoxin
-	121382..121606	-	225	NuclAT_0	-	Antitoxin
-	121382..121606	-	225	NuclAT_0	-	Antitoxin
-	121382..121606	-	225	NuclAT_0	-	Antitoxin
FQR64_RS25740	121418..121606	+	189	WP_001299721.1	hypothetical protein	-
FQR64_RS24015	121618..122337	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
FQR64_RS24020	122334..122768	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
FQR64_RS24025	122823..124781	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
FQR64_RS24030	124840..125073	-	234	WP_000006004.1	DUF905 domain-containing protein	-
FQR64_RS24035	125129..125656	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
FQR64_RS24055	126129..126395	-	267	WP_072254135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaTEM-1C / dfrA14 / qnrS1 / floR / sitABCD	iroB / iroC / iroD / iroE / iroN	1..146133	146133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T135970 WP_001312861.1 NZ_CP042586:c121338-121180 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T135970 NZ_CP055199:c1956034-1955660 [Shigella flexneri]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT135970 NZ_CP042586:c121606-121382 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin