Detailed information of TA system TA135969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4528792..4529394
• Replicon: chromosome
• Accession: NZ_CP042585
• Organism: Escherichia coli strain LD91-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FQR64_RS22315
• Protein ID: WP_000897305.1
• Coordinates: 4529083..4529394 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FQR64_RS22310
• Protein ID: WP_000356397.1
• Coordinates: 4528792..4529082 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQR64_RS22285	4524718..4525620	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FQR64_RS22290	4525617..4526252	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FQR64_RS22295	4526249..4527178	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FQR64_RS22300	4527508..4527750	-	243	WP_001086388.1	hypothetical protein	-
FQR64_RS22305	4527969..4528187	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FQR64_RS22310	4528792..4529082	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FQR64_RS22315	4529083..4529394	-	312	WP_000897305.1	hypothetical protein	Toxin
FQR64_RS22320	4529623..4530531	+	909	WP_001162704.1	alpha/beta hydrolase	-
FQR64_RS22325	4530595..4531536	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
FQR64_RS22330	4531581..4532018	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FQR64_RS22335	4532015..4532887	-	873	WP_000920770.1	virulence factor BrkB family protein	-
FQR64_RS22340	4532881..4533480	-	600	WP_001295269.1	glucose-1-phosphatase	-
FQR64_RS22345	4533579..4534364	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T135969 WP_000897305.1 NZ_CP042585:c4529394-4529083 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T135969 NZ_CP042585:c4529394-4529083 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT135969 WP_000356397.1 NZ_CP042585:c4529082-4528792 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT135969 NZ_CP042585:c4529082-4528792 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7