Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3663975..3664516 Replicon chromosome
Accession NZ_CP042585
Organism Escherichia coli strain LD91-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FQR64_RS18285 Protein ID WP_000615976.1
Coordinates 3664238..3664516 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FQR64_RS18280 Protein ID WP_000729704.1
Coordinates 3663975..3664235 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQR64_RS18255 3659758..3660543 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
FQR64_RS18260 3660488..3662227 + 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
FQR64_RS18265 3662332..3662610 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FQR64_RS18270 3662603..3662959 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FQR64_RS18275 3663016..3663789 - 774 WP_000543897.1 C40 family peptidase -
FQR64_RS18280 3663975..3664235 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FQR64_RS18285 3664238..3664516 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FQR64_RS18290 3664672..3665412 + 741 WP_001225679.1 murein L,D-transpeptidase -
FQR64_RS18295 3665383..3666150 - 768 WP_000333380.1 class II glutamine amidotransferase -
FQR64_RS18300 3666356..3666934 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T135965 WP_000615976.1 NZ_CP042585:3664238-3664516 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T135965 NZ_CP042585:3664238-3664516 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT135965 WP_000729704.1 NZ_CP042585:3663975-3664235 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT135965 NZ_CP042585:3663975-3664235 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References