Detailed information of TA system TA135931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31255..31985
• Replicon: chromosome
• Accession: NZ_CP042578
• Organism: Enterobacter kobei strain C16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0PHU0
• Locus tag: FR825_RS00160
• Protein ID: WP_014881928.1
• Coordinates: 31255..31569 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR825_RS00165
• Protein ID: WP_023331687.1
• Coordinates: 31566..31985 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR825_RS00130	26920..28008	+	1089	WP_014881924.1	cellulase family glycosylhydrolase	-
FR825_RS00135	27911..29095	-	1185	WP_014881925.1	multidrug efflux MFS transporter EmrD	-
FR825_RS00140	29271..30104	-	834	WP_014881926.1	DMT family transporter	-
FR825_RS00145	30167..30613	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
FR825_RS00150	30678..30767	-	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
FR825_RS00160	31255..31569	+	315	WP_014881928.1	type II toxin-antitoxin system HigB family toxin	Toxin
FR825_RS00165	31566..31985	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
FR825_RS00170	32135..32233	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
FR825_RS00175	32340..34028	+	1689	WP_014881930.1	acetolactate synthase large subunit	-
FR825_RS00180	34032..34319	+	288	WP_010426528.1	acetolactate synthase small subunit	-
FR825_RS00185	34402..34995	+	594	WP_014881931.1	transcriptional regulator UhpA	-
FR825_RS00190	34992..36496	+	1505	Protein_36	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12437.60 Da
• Isoelectric Point: 10.2825

>T135931 WP_014881928.1 NZ_CP042578:31255-31569 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCPTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T135931 NZ_CP042578:31255-31569 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCCCGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT135931 WP_023331687.1 NZ_CP042578:31566-31985 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT135931 NZ_CP042578:31566-31985 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2J0PHU0