Detailed information of TA system TA135896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2908..3493
• Replicon: plasmid pE47_004
• Accession: NZ_CP042560
• Organism: Acinetobacter baumannii strain E47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FR761_RS20650
• Protein ID: WP_000897310.1
• Coordinates: 3173..3493 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR761_RS20645
• Protein ID: WP_000369780.1
• Coordinates: 2908..3180 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR761_RS20630	1..951	+	951	WP_001208778.1	replication initiation protein RepM	-
FR761_RS20635	944..1519	+	576	WP_086231552.1	helix-turn-helix domain-containing protein	-
FR761_RS20950	1540..1683	+	144	WP_004786602.1	hypothetical protein	-
FR761_RS20640	1896..2423	+	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
FR761_RS20645	2908..3180	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
FR761_RS20650	3173..3493	-	321	WP_000897310.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR761_RS20655	3680..3877	-	198	WP_000476222.1	hypothetical protein	-
FR761_RS20660	3944..4171	-	228	WP_000921865.1	hypothetical protein	-
FR761_RS20665	4269..4484	-	216	WP_000071892.1	cold-shock protein	-
FR761_RS20670	4810..5241	-	432	WP_000390052.1	hypothetical protein	-
FR761_RS20675	5259..5525	-	267	WP_006581723.1	hypothetical protein	-
FR761_RS20680	5735..7171	+	1437	WP_057050431.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7703	7703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (40-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12208.02 Da
• Isoelectric Point: 8.8648

>T135896 WP_000897310.1 NZ_CP042560:c3493-3173 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGNLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T135896 NZ_CP042560:c3493-3173 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCAACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT135896 WP_000369780.1 NZ_CP042560:c3180-2908 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT135896 NZ_CP055181:2294864-2295130 [Shigella flexneri]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA