Detailed information of TA system TA135863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 46138..46865
• Replicon: plasmid pC52_001
• Accession: NZ_CP042546
• Organism: Klebsiella michiganensis strain C52

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: FR848_RS28205
• Protein ID: WP_004118600.1
• Coordinates: 46138..46449 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: FR848_RS28210
• Protein ID: WP_000990392.1
• Coordinates: 46446..46865 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR848_RS28185	42652..43371	+	720	WP_001102107.1	(Fe-S)-binding protein	-
FR848_RS28190	43382..44809	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
FR848_RS28195	44802..45497	+	696	WP_004118595.1	lactate utilization protein C	-
FR848_RS28200	45547..45927	-	381	Protein_45	gluconate permease	-
FR848_RS28205	46138..46449	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
FR848_RS28210	46446..46865	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
FR848_RS28215	46902..48104	-	1203	WP_003031541.1	hypothetical protein	-
FR848_RS28220	48097..48426	-	330	WP_004118613.1	hypothetical protein	-
FR848_RS28225	48423..49082	-	660	WP_000078540.1	hypothetical protein	-
FR848_RS30715	49506..50099	-	594	WP_044348674.1	hypothetical protein	-
FR848_RS28240	50415..50768	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FR848_RS28245	50816..51178	+	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..195216	195216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T135863 WP_004118600.1 NZ_CP042546:46138-46449 [Klebsiella michiganensis]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T135863 NZ_CP042546:46138-46449 [Klebsiella michiganensis]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT135863 WP_000990392.1 NZ_CP042546:46446-46865 [Klebsiella michiganensis]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT135863 NZ_CP042546:46446-46865 [Klebsiella michiganensis]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGATCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4