Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 401169..401819 Replicon chromosome
Accession NZ_CP042545
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag FR848_RS01880 Protein ID WP_014227314.1
Coordinates 401169..401510 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag FR848_RS01885 Protein ID WP_014227313.1
Coordinates 401520..401819 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS01860 397161..398480 + 1320 WP_014227316.1 MFS transporter -
FR848_RS01865 398626..400017 + 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
FR848_RS01875 400466..400918 + 453 WP_014839842.1 DUF1198 domain-containing protein -
FR848_RS01880 401169..401510 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FR848_RS01885 401520..401819 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
FR848_RS01890 401938..403119 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
FR848_RS01895 403152..403961 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
FR848_RS01900 403971..405074 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
FR848_RS01905 405215..405934 + 720 WP_032718745.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T135847 WP_014227314.1 NZ_CP042545:401169-401510 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T135847 NZ_CP042545:401169-401510 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT135847 WP_014227313.1 NZ_CP042545:401520-401819 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT135847 NZ_CP042545:401520-401819 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AGCCCACCATTGCTAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References