Detailed information of TA system TA135828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 117458..118182
• Replicon: plasmid pC9_001
• Accession: NZ_CP042531
• Organism: Klebsiella aerogenes strain C9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FR830_RS24970
• Protein ID: WP_045379379.1
• Coordinates: 117871..118182 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR830_RS24965
• Protein ID: WP_045379376.1
• Coordinates: 117458..117874 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR830_RS24935	112647..112859	-	213	WP_001446195.1	cold shock-like protein CspF	-
FR830_RS24940	113425..113780	-	356	Protein_98	transposase	-
FR830_RS24945	113807..114089	-	283	Protein_99	P2 family phage major capsid protein	-
FR830_RS24950	114325..115005	+	681	WP_192797125.1	hypothetical protein	-
FR830_RS24955	115157..115402	+	246	WP_151586275.1	transposase	-
FR830_RS24960	115424..115738	-	315	WP_151586013.1	hypothetical protein	-
FR830_RS24965	117458..117874	-	417	WP_045379376.1	helix-turn-helix domain-containing protein	Antitoxin
FR830_RS24970	117871..118182	-	312	WP_045379379.1	type II toxin-antitoxin system HigB family toxin	Toxin
FR830_RS24975	118369..118686	-	318	WP_192797136.1	helix-turn-helix domain-containing protein	-
FR830_RS24980	118834..119712	-	879	WP_151586019.1	incFII family plasmid replication initiator RepA	-
FR830_RS24985	119917..120462	-	546	WP_151586022.1	phospholipase D family protein	-
FR830_RS24990	120651..121352	-	702	WP_151586025.1	type-F conjugative transfer system pilin acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..215014	215014
	-	flank	IS/Tn	-	-	113541..113780	239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12444.26 Da
• Isoelectric Point: 10.0952

>T135828 WP_045379379.1 NZ_CP042531:c118182-117871 [Klebsiella aerogenes]
MHVISKEPFEEATRRFPNDAVALKALYRLVREKDFASPVELRSVFPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHITTHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T135828 NZ_CP042531:c118182-117871 [Klebsiella aerogenes]
ATGCATGTCATATCTAAAGAACCCTTTGAAGAAGCTACGAGACGGTTTCCGAATGATGCCGTAGCGCTTAAAGCTTTGTA
TCGACTTGTGCGAGAAAAGGATTTCGCTTCACCAGTTGAACTGAGGTCAGTGTTTCCGAGCCTTGATAACTTTAAGTACA
GAAACAAATGGTGGGTTCTGGATGTTGGTGGCAATAATTTACGTGTAATTGCCTATATTAATTTTGTAAATAAGCGATTT
TACGTAAAGCATATTACCACTCATGCTGAGTACGATAAATTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15158.19 Da
• Isoelectric Point: 4.2694

>AT135828 WP_045379376.1 NZ_CP042531:c117874-117458 [Klebsiella aerogenes]
MIATTKAIEATKELVAAVPFLGGSATETDYRDALALVDYLIENDDENPLIDFLASKISEYEDNCESFADFNNSIAEMPVG
VALLRTIIDQHKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSARFGVKAEWFL

Nucleotide

• Length: 417 bp

>AT135828 NZ_CP042531:c117874-117458 [Klebsiella aerogenes]
ATGATTGCTACAACGAAAGCTATCGAAGCAACTAAAGAACTGGTAGCCGCAGTACCTTTCCTGGGGGGCAGTGCCACCGA
AACTGATTACCGTGACGCTCTGGCACTGGTGGATTATCTAATTGAAAATGATGATGAAAACCCGCTGATTGATTTCCTTG
CCAGTAAAATTTCTGAGTATGAGGACAACTGTGAATCTTTCGCCGATTTCAACAATTCCATTGCTGAAATGCCAGTTGGT
GTAGCCTTGCTGCGTACGATTATCGATCAACATAAACTGTCATACTCTGACCTGAAAGAGGAGATTGGGTCAAAGTCGCT
GGTAAGTCAGATCCTGTCCGGACAACGTTCATTGACAATTGCTCATATTAAGGCACTTTCCGCACGTTTTGGTGTTAAAG
CTGAATGGTTTCTTTAA