Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4158629..4159295 Replicon chromosome
Accession NZ_CP042517
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag FR815_RS20445 Protein ID WP_003847996.1
Coordinates 4158629..4158946 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag FR815_RS20450 Protein ID WP_003837894.1
Coordinates 4158999..4159295 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS20430 4153799..4154677 + 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -
FR815_RS20435 4154787..4156505 - 1719 WP_016151099.1 DUF4153 domain-containing protein -
FR815_RS20440 4156884..4158506 + 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
FR815_RS20445 4158629..4158946 + 318 WP_003847996.1 hypothetical protein Toxin
FR815_RS20450 4158999..4159295 + 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
FR815_RS20455 4159352..4160704 - 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
FR815_RS20460 4160701..4161420 - 720 WP_001157751.1 two-component system response regulator OmpR -
FR815_RS20465 4161646..4162119 + 474 WP_003023524.1 transcription elongation factor GreB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T135786 WP_003847996.1 NZ_CP042517:4158629-4158946 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T135786 NZ_CP042517:4158629-4158946 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT135786 WP_003837894.1 NZ_CP042517:4158999-4159295 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT135786 NZ_CP042517:4158999-4159295 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References