Detailed information of TA system TA135735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2893205..2893851
• Replicon: chromosome
• Accession: NZ_CP042499
• Organism: Enterobacter sp. E76

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FR762_RS13705
• Protein ID: WP_151604128.1
• Coordinates: 2893205..2893558 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR762_RS13710
• Protein ID: WP_151604130.1
• Coordinates: 2893555..2893851 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR762_RS13695	2888629..2891751	-	3123	WP_144130447.1	MdtB/MuxB family multidrug efflux RND transporter permease subunit	-
FR762_RS13700	2891751..2892977	-	1227	WP_151604126.1	MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit	-
FR762_RS13705	2893205..2893558	+	354	WP_151604128.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR762_RS13710	2893555..2893851	+	297	WP_151604130.1	XRE family transcriptional regulator	Antitoxin
FR762_RS13715	2893874..2895226	-	1353	WP_151605632.1	molecular chaperone	-
FR762_RS13720	2895358..2896197	+	840	WP_151604132.1	DNA-3-methyladenine glycosylase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13612.66 Da
• Isoelectric Point: 9.2409

>T135735 WP_151604128.1 NZ_CP042499:2893205-2893558 [Enterobacter sp. E76]
MTWTVVFTPEFEQWLYDQELGLQDRVQASLVPLEAAGPALGRPMVDTLAGSRYPNMKELRVQYGGDPWRILFAFDYNREA
VMLYGGKKVGKKRFYTSLIARVDAIFERYLARRKDQE

Nucleotide

• Length: 354 bp

>T135735 NZ_CP042499:2893205-2893558 [Enterobacter sp. E76]
ATGACATGGACGGTTGTCTTTACGCCAGAGTTTGAACAATGGCTGTATGACCAGGAACTGGGATTACAGGATCGCGTGCA
AGCCTCTTTAGTCCCACTTGAAGCTGCCGGCCCAGCTTTGGGTCGCCCTATGGTTGATACGCTTGCCGGTTCACGTTATC
CCAATATGAAAGAGCTTCGCGTGCAGTATGGTGGTGATCCCTGGAGGATATTGTTTGCATTTGATTACAATCGAGAAGCG
GTAATGCTTTATGGCGGAAAGAAAGTAGGGAAAAAGCGCTTTTATACCTCGTTAATTGCGCGAGTTGATGCAATTTTTGA
ACGTTATCTTGCCCGTAGAAAGGATCAGGAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10959.69 Da
• Isoelectric Point: 5.9126

>AT135735 WP_151604130.1 NZ_CP042499:2893555-2893851 [Enterobacter sp. E76]
MKTLQQHLAELSPERREKIMRMADEIAAENGLPTIREMRAMSQQELAERLGVTQPAVAAMERRGDDIKVSTLKRYAEALG
GELKISIELPEGSRVISL

Nucleotide

• Length: 297 bp

>AT135735 NZ_CP042499:2893555-2893851 [Enterobacter sp. E76]
ATGAAAACATTACAGCAACATCTCGCAGAACTTTCTCCTGAACGTCGTGAGAAAATTATGCGTATGGCGGATGAAATCGC
TGCCGAAAACGGCTTACCGACCATCAGAGAAATGCGCGCCATGTCGCAGCAGGAGCTTGCCGAAAGGCTGGGCGTAACCC
AGCCTGCGGTGGCCGCCATGGAGCGGCGCGGAGATGATATTAAAGTCTCGACGTTGAAACGTTACGCAGAAGCCCTGGGC
GGTGAATTGAAGATCAGCATCGAACTGCCCGAAGGAAGCAGAGTGATTTCACTCTGA