Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1258924..1259651 Replicon chromosome
Accession NZ_CP042488
Organism Enterobacter hormaechei strain C15

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag FR808_RS06040 Protein ID WP_022650547.1
Coordinates 1259340..1259651 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag FR808_RS06035 Protein ID WP_022650546.1
Coordinates 1258924..1259343 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR808_RS06020 1255996..1257117 - 1122 WP_003858868.1 glutamate--cysteine ligase -
FR808_RS06025 1257223..1258113 + 891 WP_022650544.1 oxidoreductase -
FR808_RS06030 1258166..1258600 - 435 WP_022650545.1 hypothetical protein -
FR808_RS06035 1258924..1259343 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
FR808_RS06040 1259340..1259651 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
FR808_RS25460 1260344..1260763 + 420 WP_032645895.1 hypothetical protein -
FR808_RS06050 1260876..1261331 - 456 WP_022650549.1 hypothetical protein -
FR808_RS06055 1261614..1262495 - 882 WP_022650550.1 LysR family transcriptional regulator -
FR808_RS06060 1262601..1263380 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T135713 WP_022650547.1 NZ_CP042488:c1259651-1259340 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T135713 NZ_CP042488:c1259651-1259340 [Enterobacter hormaechei]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT135713 WP_022650546.1 NZ_CP042488:c1259343-1258924 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT135713 NZ_CP042488:c1259343-1258924 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References