Detailed information of TA system TA135644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3195610..3196225
• Replicon: chromosome
• Accession: NZ_CP042382
• Organism: Pistricoccus aurantiacus strain CBA4606

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FGL86_RS15110
• Protein ID: WP_147186231.1
• Coordinates: 3195902..3196225 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FGL86_RS15105
• Protein ID: WP_147185467.1
• Coordinates: 3195610..3195909 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL86_RS15080	3191607..3192335	-	729	WP_186764419.1	PilZ domain-containing protein	-
FGL86_RS15085	3192903..3194099	+	1197	WP_147185459.1	FliC/FljB family flagellin	-
FGL86_RS15090	3194192..3194851	+	660	WP_147185461.1	hypothetical protein	-
FGL86_RS15095	3195048..3195272	+	225	WP_147185463.1	hypothetical protein	-
FGL86_RS15100	3195294..3195569	-	276	WP_147185465.1	helix-turn-helix domain-containing protein	-
FGL86_RS15105	3195610..3195909	-	300	WP_147185467.1	helix-turn-helix domain-containing protein	Antitoxin
FGL86_RS15110	3195902..3196225	-	324	WP_147186231.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGL86_RS15115	3196268..3196465	-	198	WP_147185469.1	transposase	-
FGL86_RS15120	3196465..3196857	-	393	WP_147185471.1	type II toxin-antitoxin system VapC family toxin	-
FGL86_RS15125	3196930..3197421	-	492	WP_147186232.1	pilin	-
FGL86_RS15130	3197657..3199411	+	1755	WP_147185472.1	type IV-A pilus assembly ATPase PilB	-
FGL86_RS15135	3199534..3200763	+	1230	WP_147185474.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (32-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11937.93 Da
• Isoelectric Point: 8.4538

>T135644 WP_147186231.1 NZ_CP042382:c3196225-3195902 [Pistricoccus aurantiacus]
MVFIELPIFIRCANALFTDADLTELQITLLENPAAGDLIPGGKGLRKIRAPLPGRGKRGGARVIYYYWVSKEKCYLVYAY
AKNVAADLTKDQLHRLAAVIEAEVRDE

Nucleotide

• Length: 324 bp

>T135644 NZ_CP042382:c3196225-3195902 [Pistricoccus aurantiacus]
ATGGTCTTCATCGAACTGCCCATTTTTATTCGCTGCGCTAATGCACTATTTACAGATGCAGATTTGACCGAGCTTCAAAT
TACGTTGCTCGAGAATCCAGCAGCAGGCGATCTTATTCCTGGCGGCAAGGGATTGCGCAAAATACGCGCTCCTTTACCGG
GGCGAGGAAAACGAGGTGGCGCTCGAGTCATTTATTATTATTGGGTATCGAAAGAGAAATGCTATCTCGTATATGCCTAT
GCCAAGAATGTGGCAGCAGACTTGACGAAAGATCAATTACACCGTTTGGCTGCCGTCATTGAAGCGGAGGTGCGAGATGA
ATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11373.09 Da
• Isoelectric Point: 9.9177

>AT135644 WP_147185467.1 NZ_CP042382:c3195909-3195610 [Pistricoccus aurantiacus]
MNEQSFDELLESVRDMGHHMRGELVQGVRTREFPEPDVKAIRERTGLSQTRFAYLIGVKPKTLQNWEQKRVRPTGPARAL
LKIIEVNPDALAAIHVREV

Nucleotide

• Length: 300 bp

>AT135644 NZ_CP042382:c3195909-3195610 [Pistricoccus aurantiacus]
ATGAATGAACAGTCTTTTGACGAACTGCTGGAAAGCGTTCGAGATATGGGTCATCACATGCGTGGTGAACTGGTGCAGGG
AGTTCGGACTCGAGAATTTCCTGAACCAGACGTGAAAGCTATCCGCGAGCGTACCGGCTTATCACAGACTCGATTCGCCT
ATTTAATAGGCGTGAAACCGAAGACGCTCCAGAACTGGGAACAGAAGCGGGTTCGTCCAACGGGTCCTGCGCGAGCACTG
CTGAAAATCATTGAGGTAAATCCGGACGCTTTGGCTGCGATTCATGTCCGAGAGGTGTAG