Detailed information of TA system TA135628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8469..9120
• Replicon: plasmid pC54_002
• Accession: NZ_CP042366
• Organism: Acinetobacter pittii strain C54

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FR838_RS20300
• Protein ID: WP_000269910.1
• Coordinates: 8469..8777 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR838_RS20305
• Protein ID: WP_001140619.1
• Coordinates: 8818..9120 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR838_RS20260	3927..4361	+	435	WP_106405795.1	hypothetical protein	-
FR838_RS20270	5543..5962	+	420	Protein_8	IS3 family transposase	-
FR838_RS20275	6001..6366	+	366	Protein_9	hypothetical protein	-
FR838_RS20280	6623..6868	+	246	WP_000246757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FR838_RS20285	6858..7151	+	294	WP_000221360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FR838_RS20290	7338..7454	+	117	Protein_12	transposase	-
FR838_RS20300	8469..8777	+	309	WP_000269910.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR838_RS20305	8818..9120	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
FR838_RS20310	9113..9322	+	210	WP_000069470.1	hypothetical protein	-
FR838_RS20315	9432..9659	-	228	WP_000555567.1	hypothetical protein	-
FR838_RS20325	10425..10728	-	304	Protein_17	helix-turn-helix domain-containing protein	-
FR838_RS20330	10721..11077	-	357	WP_000269908.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FR838_RS20345	13062..13550	+	489	Protein_20	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76008	76008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-102)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11742.64 Da
• Isoelectric Point: 9.0002

>T135628 WP_000269910.1 NZ_CP042366:8469-8777 [Acinetobacter pittii]
MWTVITTDLFNEWLVQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIAD

Nucleotide

• Length: 309 bp

>T135628 NZ_CP042366:8469-8777 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGACGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT135628 WP_001140619.1 NZ_CP042366:8818-9120 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT135628 NZ_CP042366:8818-9120 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTGGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTCTAA