Detailed information of TA system TA135626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 110366..110951
• Replicon: plasmid pC54_001
• Accession: NZ_CP042365
• Organism: Acinetobacter pittii strain C54

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: FR838_RS19565
• Protein ID: WP_000897307.1
• Coordinates: 110366..110686 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR838_RS19570
• Protein ID: WP_000369781.1
• Coordinates: 110679..110951 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR838_RS19535	105839..106228	-	390	Protein_112	hypothetical protein	-
FR838_RS19540	106377..106679	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FR838_RS19545	106680..106961	+	282	WP_000985609.1	putative addiction module antidote protein	-
FR838_RS19550	107041..107460	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FR838_RS19555	107583..108748	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
FR838_RS19560	108901..110235	+	1335	WP_033917544.1	ISNCY family transposase	-
FR838_RS19565	110366..110686	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR838_RS19570	110679..110951	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
FR838_RS19575	111444..112919	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FR838_RS19580	112975..113859	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FR838_RS19585	114041..114745	-	705	WP_004897650.1	IS6-like element IS1006 family transposase	-
FR838_RS19590	114678..115553	-	876	WP_114190151.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / aph(3')-VIa / sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19 / blaOXA-58	-	1..256887	256887
	-	inside	IScluster/Tn	sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19	-	98009..133938	35929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T135626 WP_000897307.1 NZ_CP042365:110366-110686 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T135626 NZ_CP042365:110366-110686 [Acinetobacter pittii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT135626 WP_000369781.1 NZ_CP042365:110679-110951 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT135626 NZ_CP042365:110679-110951 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3