Detailed information of TA system TA135516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 327671..328365
• Replicon: chromosome
• Accession: NZ_CP042352
• Organism: Escherichia coli strain RM9513

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FS836_RS02025
• Protein ID: WP_001263493.1
• Coordinates: 327967..328365 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FS836_RS02020
• Protein ID: WP_000554757.1
• Coordinates: 327671..327964 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS836_RS02000 (323303)	323303..323800	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FS836_RS02005 (324024)	324024..325736	-	1713	Protein_294	flagellar biosynthesis protein FlhA	-
FS836_RS02010 (325708)	325708..326493	+	786	WP_192892826.1	putative lateral flagellar export/assembly protein LafU	-
FS836_RS02015 (326564)	326564..327619	+	1056	WP_001226164.1	DNA polymerase IV	-
FS836_RS02020 (327671)	327671..327964	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FS836_RS02025 (327967)	327967..328365	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FS836_RS02030 (328375)	328375..328827	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FS836_RS02035 (329073)	329073..329276	+	204	Protein_300	RtcB family protein	-
FS836_RS02040 (329275)	329275..329796	+	522	Protein_301	peptide chain release factor H	-
FS836_RS02045 (329853)	329853..331310	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FS836_RS02050 (331571)	331571..332029	+	459	WP_061349133.1	xanthine phosphoribosyltransferase	-
- (332625)	332625..332705	+	81	NuclAT_9	-	-
- (332625)	332625..332705	+	81	NuclAT_9	-	-
- (332625)	332625..332705	+	81	NuclAT_9	-	-
- (332625)	332625..332705	+	81	NuclAT_9	-	-
FS836_RS02055 (332121)	332121..333365	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	312223..329796	17573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T135516 WP_001263493.1 NZ_CP042352:327967-328365 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T135516 NZ_CP042352:327967-328365 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT135516 WP_000554757.1 NZ_CP042352:327671-327964 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT135516 NZ_CP042352:327671-327964 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6