Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 321627..322168 Replicon chromosome
Accession NZ_CP042352
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag FS836_RS01985 Protein ID WP_000615979.1
Coordinates 321627..321905 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FS836_RS01990 Protein ID WP_000729704.1
Coordinates 321908..322168 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS01970 (319209) 319209..319787 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FS836_RS01975 (319993) 319993..320760 + 768 WP_000333379.1 class II glutamine amidotransferase -
FS836_RS01980 (320731) 320731..321471 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FS836_RS01985 (321627) 321627..321905 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FS836_RS01990 (321908) 321908..322168 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FS836_RS01995 (322378) 322378..323127 + 750 WP_061349132.1 C40 family peptidase -
FS836_RS02000 (323303) 323303..323800 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FS836_RS02005 (324024) 324024..325736 - 1713 Protein_294 flagellar biosynthesis protein FlhA -
FS836_RS02010 (325708) 325708..326493 + 786 WP_192892826.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 312223..329796 17573


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T135515 WP_000615979.1 NZ_CP042352:c321905-321627 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T135515 NZ_CP042352:c321905-321627 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT135515 WP_000729704.1 NZ_CP042352:c322168-321908 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT135515 NZ_CP042352:c322168-321908 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTACCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References