Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2300638..2301140 Replicon chromosome
Accession NZ_CP042350
Organism Escherichia coli strain RM10410

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FS611_RS11810 Protein ID WP_000767829.1
Coordinates 2300638..2300892 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FS611_RS11815 Protein ID WP_001259255.1
Coordinates 2300889..2301140 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS611_RS11780 2295653..2295880 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FS611_RS11785 2295894..2296952 - 1059 WP_000492310.1 transport protein YeeE -
FS611_RS11790 2297131..2298489 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FS611_RS11795 2298479..2298541 - 63 WP_010723108.1 membrane protein YoeI -
FS611_RS11800 2298756..2299685 - 930 WP_000803360.1 LysR family transcriptional regulator -
FS611_RS11805 2299731..2300555 - 825 WP_000754737.1 SDR family oxidoreductase -
FS611_RS11810 2300638..2300892 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FS611_RS11815 2300889..2301140 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FS611_RS11820 2301424..2301474 + 51 WP_001364200.1 his operon leader peptide -
FS611_RS11825 2301620..2302519 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FS611_RS11830 2302525..2303829 + 1305 WP_033801387.1 histidinol dehydrogenase -
FS611_RS11835 2303826..2304896 + 1071 WP_033801386.1 histidinol-phosphate transaminase -
FS611_RS11840 2304896..2305963 + 1068 WP_033801385.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T135501 WP_000767829.1 NZ_CP042350:c2300892-2300638 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T135501 NZ_CP042350:c2300892-2300638 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT135501 WP_001259255.1 NZ_CP042350:c2301140-2300889 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT135501 NZ_CP042350:c2301140-2300889 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References