Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 265000..265694 Replicon chromosome
Accession NZ_CP042350
Organism Escherichia coli strain RM10410

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FS611_RS01205 Protein ID WP_001263489.1
Coordinates 265296..265694 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FS611_RS01200 Protein ID WP_000554758.1
Coordinates 265000..265293 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS611_RS01180 260632..261129 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FS611_RS01185 261353..263092 - 1740 WP_148050495.1 flagellar biosynthesis protein FlhA -
FS611_RS01190 263037..263822 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FS611_RS01195 263893..264948 + 1056 WP_001226164.1 DNA polymerase IV -
FS611_RS01200 265000..265293 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FS611_RS01205 265296..265694 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FS611_RS01210 265704..266135 + 432 WP_148050496.1 GNAT family N-acetyltransferase -
FS611_RS01220 266205..267413 + 1209 WP_040077378.1 IS4-like element ISVsa5 family transposase -
FS611_RS01225 267812..268018 + 207 Protein_236 RtcB family protein -
FS611_RS01230 268014..268535 + 522 Protein_237 peptide chain release factor H -
FS611_RS01235 268592..270049 - 1458 WP_040076790.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 266205..267413 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T135488 WP_001263489.1 NZ_CP042350:265296-265694 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T135488 NZ_CP042350:265296-265694 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT135488 WP_000554758.1 NZ_CP042350:265000-265293 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT135488 NZ_CP042350:265000-265293 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References