Detailed information of TA system TA135433

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117022..117255
• Replicon: plasmid pCTXM-GZ04
• Accession: NZ_CP042337
• Organism: Escherichia coli strain GZ04-0086

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E3B71_RS26310
• Protein ID: WP_001312861.1
• Coordinates: 117022..117180 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	117224..117255 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3B71_RS26275	112396..113085	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
E3B71_RS26280	113272..113655	-	384	WP_001151524.1	relaxosome protein TraM	-
E3B71_RS26285	113976..114578	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
E3B71_RS26290	114875..115696	-	822	WP_001234426.1	DUF945 domain-containing protein	-
E3B71_RS26295	115814..116101	-	288	WP_000107535.1	hypothetical protein	-
E3B71_RS26310	117022..117180	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	117224..117255	-	32	NuclAT_1	-	Antitoxin
-	117224..117255	-	32	NuclAT_1	-	Antitoxin
-	117224..117255	-	32	NuclAT_1	-	Antitoxin
-	117224..117255	-	32	NuclAT_1	-	Antitoxin
-	118697..118894	-	198	NuclAT_0	-	-
-	118697..118894	-	198	NuclAT_0	-	-
-	118697..118894	-	198	NuclAT_0	-	-
-	118697..118894	-	198	NuclAT_0	-	-
E3B71_RS27665	118706..118894	+	189	WP_001299721.1	hypothetical protein	-
E3B71_RS26320	118906..119625	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
E3B71_RS26325	119622..120056	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
E3B71_RS26330	120111..120308	-	198	Protein_142	hypothetical protein	-
E3B71_RS26335	120336..120569	-	234	WP_000005990.1	DUF905 family protein	-
E3B71_RS26340	120637..121176	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
E3B71_RS26345	121202..121408	-	207	WP_000275856.1	hypothetical protein	-
E3B71_RS26360	121818..122024	-	207	WP_001774176.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..159610	159610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T135433 WP_001312861.1 NZ_CP042337:c117180-117022 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T135433 NZ_CP042337:c117180-117022 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT135433 NZ_CP042337:c117255-117224 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin