Detailed information of TA system TA135429

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76955..77209
• Replicon: plasmid pCTXM-GZ04
• Accession: NZ_CP042337
• Organism: Escherichia coli strain GZ04-0086

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: E3B71_RS26055
• Protein ID: WP_015387399.1
• Coordinates: 76955..77161 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77148..77209 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3B71_RS26020	72202..73113	-	912	WP_000440183.1	carbamate kinase	-
E3B71_RS26025	73124..74344	-	1221	WP_000410951.1	arginine deiminase	-
E3B71_RS26030	75237..75719	+	483	Protein_87	VENN motif pre-toxin domain-containing protein	-
E3B71_RS26035	75665..76111	-	447	Protein_88	incFII family plasmid replication initiator RepA	-
E3B71_RS26040	76104..76178	-	75	WP_032336874.1	RepA leader peptide Tap	-
E3B71_RS26050	76414..76671	-	258	WP_000083833.1	replication regulatory protein RepA	-
E3B71_RS26055	76955..77161	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77148..77209	+	62	NuclAT_2	-	Antitoxin
-	77148..77209	+	62	NuclAT_2	-	Antitoxin
-	77148..77209	+	62	NuclAT_2	-	Antitoxin
-	77148..77209	+	62	NuclAT_2	-	Antitoxin
E3B71_RS26065	77348..77530	-	183	WP_000968309.1	hypothetical protein	-
E3B71_RS26070	77631..78247	+	617	Protein_93	IS1 family transposase	-
E3B71_RS26075	78285..79856	-	1572	WP_023149734.1	IS66 family transposase	-
E3B71_RS26080	79876..80223	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
E3B71_RS26085	80223..80900	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
E3B71_RS27660	80955..81044	+	90	Protein_97	IS1 family transposase	-
E3B71_RS26100	81345..81557	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..159610	159610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T135429 WP_015387399.1 NZ_CP042337:c77161-76955 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T135429 NZ_CP042337:c77161-76955 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT135429 NZ_CP042337:77148-77209 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin