Detailed information of TA system TA135427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4118972..4119666
• Replicon: chromosome
• Accession: NZ_CP042336
• Organism: Escherichia coli strain GZ04-0086

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: E3B71_RS22155
• Protein ID: WP_001263489.1
• Coordinates: 4119268..4119666 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: E3B71_RS22150
• Protein ID: WP_000554758.1
• Coordinates: 4118972..4119265 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3B71_RS22130	4114604..4115101	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
E3B71_RS22135	4115325..4117037	-	1713	Protein_3889	flagellar biosynthesis protein FlhA	-
E3B71_RS22140	4117009..4117794	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
E3B71_RS22145	4117865..4118920	+	1056	WP_001226164.1	DNA polymerase IV	-
E3B71_RS22150	4118972..4119265	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3B71_RS22155	4119268..4119666	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3B71_RS22160	4119676..4120128	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
E3B71_RS22165	4120446..4120652	+	207	Protein_3895	RtcB family protein	-
E3B71_RS22170	4120648..4121169	+	522	Protein_3896	peptide chain release factor H	-
E3B71_RS22175	4121226..4122683	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
E3B71_RS22180	4122944..4123402	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	4123998..4124078	+	81	NuclAT_11	-	-
-	4123998..4124078	+	81	NuclAT_11	-	-
-	4123998..4124078	+	81	NuclAT_11	-	-
-	4123998..4124078	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T135427 WP_001263489.1 NZ_CP042336:4119268-4119666 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T135427 NZ_CP042336:4119268-4119666 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT135427 WP_000554758.1 NZ_CP042336:4118972-4119265 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT135427 NZ_CP042336:4118972-4119265 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3