Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1226843..1227345 Replicon chromosome
Accession NZ_CP042336
Organism Escherichia coli strain GZ04-0086

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3B71_RS08215 Protein ID WP_000767829.1
Coordinates 1226843..1227097 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3B71_RS08220 Protein ID WP_001259255.1
Coordinates 1227094..1227345 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3B71_RS08185 1221858..1222085 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
E3B71_RS08190 1222099..1223157 - 1059 WP_000492321.1 transport protein YeeE -
E3B71_RS08195 1223336..1224694 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3B71_RS08200 1224684..1224746 - 63 WP_010723108.1 membrane protein YoeI -
E3B71_RS08205 1224961..1225890 - 930 WP_000803351.1 LysR family transcriptional regulator -
E3B71_RS08210 1225936..1226760 - 825 WP_000754749.1 SDR family oxidoreductase -
E3B71_RS08215 1226843..1227097 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3B71_RS08220 1227094..1227345 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3B71_RS08225 1227629..1227679 + 51 WP_001364200.1 his operon leader peptide -
E3B71_RS08230 1227825..1228724 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
E3B71_RS08235 1228730..1230034 + 1305 WP_001446927.1 histidinol dehydrogenase -
E3B71_RS08240 1230031..1231101 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
E3B71_RS08245 1231101..1232168 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T135417 WP_000767829.1 NZ_CP042336:c1227097-1226843 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T135417 NZ_CP042336:c1227097-1226843 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT135417 WP_001259255.1 NZ_CP042336:c1227345-1227094 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT135417 NZ_CP042336:c1227345-1227094 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References