Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5108730..5109232 Replicon chromosome
Accession NZ_CP042298
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FRV13_RS27230 Protein ID WP_000767829.1
Coordinates 5108978..5109232 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FRV13_RS27225 Protein ID WP_001259255.1
Coordinates 5108730..5108981 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS27200 5103907..5104974 - 1068 WP_001567686.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FRV13_RS27205 5104974..5106044 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FRV13_RS27210 5106041..5107345 - 1305 WP_001567685.1 histidinol dehydrogenase -
FRV13_RS27215 5107351..5108250 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FRV13_RS27220 5108396..5108446 - 51 WP_001364200.1 his operon leader peptide -
FRV13_RS27225 5108730..5108981 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FRV13_RS27230 5108978..5109232 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FRV13_RS27235 5109315..5110139 + 825 WP_001567684.1 SDR family oxidoreductase -
FRV13_RS27240 5110185..5111114 + 930 WP_000803351.1 LysR family transcriptional regulator -
FRV13_RS27245 5111329..5111391 + 63 WP_010723108.1 membrane protein YoeI -
FRV13_RS27250 5111381..5112739 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FRV13_RS27255 5112918..5113976 + 1059 WP_000492305.1 transport protein YeeE -
FRV13_RS27260 5113990..5114217 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T135190 WP_000767829.1 NZ_CP042298:5108978-5109232 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T135190 NZ_CP042298:5108978-5109232 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT135190 WP_001259255.1 NZ_CP042298:5108730-5108981 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT135190 NZ_CP042298:5108730-5108981 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References