Detailed information of TA system TA135170

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1540739..1541110
• Replicon: chromosome
• Accession: NZ_CP042298
• Organism: Escherichia coli strain RM9088

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: FRV13_RS09230
• Protein ID: WP_042966159.1
• Coordinates: 1540916..1541110 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1540739..1540917 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRV13_RS09205	1536694..1538067	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
FRV13_RS09210	1538196..1539131	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FRV13_RS09215	1539183..1540418	-	1236	WP_072034645.1	site-specific integrase	-
FRV13_RS09220	1540420..1540635	-	216	WP_000079604.1	excisionase XisR	-
FRV13_RS09225	1540735..1540923	-	189	WP_001356607.1	DUF1187 family protein	-
-	1540739..1540917	+	179	NuclAT_0	-	Antitoxin
-	1540739..1540917	+	179	NuclAT_0	-	Antitoxin
-	1540739..1540917	+	179	NuclAT_0	-	Antitoxin
-	1540739..1540917	+	179	NuclAT_0	-	Antitoxin
FRV13_RS09230	1540916..1541110	-	195	WP_042966159.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FRV13_RS09235	1541174..1542262	-	1089	WP_042966158.1	recombinase RecT	-
FRV13_RS09240	1542277..1545423	-	3147	WP_042966157.1	exodeoxyribonuclease VIII	-
FRV13_RS09245	1545525..1545800	-	276	WP_001358843.1	hypothetical protein	-
FRV13_RS09250	1545875..1546051	-	177	WP_000258918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7047.97 Da
• Isoelectric Point: 8.9492

>T135170 WP_042966159.1 NZ_CP042298:c1541110-1540916 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNDNGGVHV

Nucleotide

• Length: 195 bp

>T135170 NZ_CP042298:c1541110-1540916 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTTAAAGCTATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATGATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT135170 NZ_CP042298:1540739-1540917 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin