Detailed information of TA system TA135162

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39282..39536
• Replicon: plasmid p2RM9088
• Accession: NZ_CP042297
• Organism: Escherichia coli strain RM9088

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FRV13_RS01260
• Protein ID: WP_001362723.1
• Coordinates: 39282..39488 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	39475..39536 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRV13_RS01225	34632..35096	-	465	WP_040090730.1	hypothetical protein	-
FRV13_RS01230	35375..36415	-	1041	WP_137561113.1	hypothetical protein	-
FRV13_RS28190	36525..36713	-	189	WP_169301934.1	hypothetical protein	-
FRV13_RS01235	37418..38440	-	1023	WP_000840936.1	replication initiation protein	-
FRV13_RS01240	38433..38507	-	75	WP_032319616.1	RepA leader peptide Tap	-
FRV13_RS01250	38741..38998	-	258	WP_000083834.1	replication regulatory protein RepA	-
FRV13_RS01260	39282..39488	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	39475..39536	+	62	NuclAT_0	-	Antitoxin
-	39475..39536	+	62	NuclAT_0	-	Antitoxin
-	39475..39536	+	62	NuclAT_0	-	Antitoxin
-	39475..39536	+	62	NuclAT_0	-	Antitoxin
FRV13_RS01265	39832..40143	+	312	WP_000765906.1	hypothetical protein	-
FRV13_RS28445	40324..40398	-	75	Protein_47	endonuclease	-
FRV13_RS01275	40459..41136	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FRV13_RS01280	41136..41483	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FRV13_RS01285	41503..43074	+	1572	WP_000381395.1	IS66 family transposase	-
FRV13_RS01290	43353..43504	-	152	Protein_51	hypothetical protein	-
FRV13_RS01295	43639..44040	-	402	Protein_52	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86529	86529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T135162 WP_001362723.1 NZ_CP042297:c39488-39282 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T135162 NZ_CP042297:c39488-39282 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT135162 NZ_CP042297:39475-39536 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin