Detailed information of TA system TA135108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4870605..4871207
• Replicon: chromosome
• Accession: NZ_CP042250
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: FQU84_RS25980
• Protein ID: WP_000897302.1
• Coordinates: 4870896..4871207 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FQU84_RS25975
• Protein ID: WP_000356397.1
• Coordinates: 4870605..4870895 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS25940	4866678..4867580	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FQU84_RS25945	4867577..4868212	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FQU84_RS25950	4868209..4869138	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FQU84_RS25955	4869354..4869572	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
FQU84_RS25965	4869968..4870246	-	279	WP_001296612.1	hypothetical protein	-
FQU84_RS25970	4870308..4870520	-	213	WP_000197774.1	hypothetical protein	-
FQU84_RS25975	4870605..4870895	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FQU84_RS25980	4870896..4871207	-	312	WP_000897302.1	hypothetical protein	Toxin
FQU84_RS25985	4871436..4872344	+	909	WP_001331553.1	alpha/beta hydrolase	-
FQU84_RS25990	4872408..4873349	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FQU84_RS25995	4873394..4873831	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FQU84_RS26000	4873828..4874700	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FQU84_RS26005	4874694..4875293	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T135108 WP_000897302.1 NZ_CP042250:c4871207-4870896 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T135108 NZ_CP042250:c4871207-4870896 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT135108 WP_000356397.1 NZ_CP042250:c4870895-4870605 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT135108 NZ_CP042250:c4870895-4870605 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGTGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416