Detailed information of TA system TA135101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3908692..3909386
• Replicon: chromosome
• Accession: NZ_CP042250
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: FQU84_RS21320
• Protein ID: WP_001263500.1
• Coordinates: 3908692..3909090 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FQU84_RS21325
• Protein ID: WP_000554758.1
• Coordinates: 3909093..3909386 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS21295	3904057..3904515	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
FQU84_RS21300	3904776..3906233	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
FQU84_RS21305	3906290..3906904	-	615	WP_000602123.1	peptide chain release factor H	-
FQU84_RS21310	3906901..3908040	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
FQU84_RS21315	3908230..3908682	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
FQU84_RS21320	3908692..3909090	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FQU84_RS21325	3909093..3909386	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FQU84_RS21330	3909438..3910493	-	1056	WP_001226168.1	DNA polymerase IV	-
FQU84_RS21335	3910564..3911349	-	786	WP_061336152.1	putative lateral flagellar export/assembly protein LafU	-
FQU84_RS21340	3911294..3913033	+	1740	WP_021512212.1	flagellar biosynthesis protein FlhA	-
FQU84_RS27220	3913212..3913370	+	159	WP_014639450.1	hypothetical protein	-
FQU84_RS21345	3913453..3913950	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3908692..3929146	20454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T135101 WP_001263500.1 NZ_CP042250:c3909090-3908692 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T135101 NZ_CP042250:c3909090-3908692 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT135101 WP_000554758.1 NZ_CP042250:c3909386-3909093 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT135101 NZ_CP042250:c3909386-3909093 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3