Detailed information of TA system TA135077

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59206..59470
• Replicon: plasmid pBCE049-2
• Accession: NZ_CP042248
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: FQU84_RS01185
• Protein ID: WP_024179514.1
• Coordinates: 59318..59470 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	59206..59268 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS01170	54445..56736	-	2292	WP_001289276.1	hypothetical protein	-
FQU84_RS01175	56729..57799	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
FQU84_RS01180	57818..59026	-	1209	WP_061336178.1	IncI1-type conjugal transfer protein TrbA	-
-	59206..59268	-	63	NuclAT_0	-	Antitoxin
-	59206..59268	-	63	NuclAT_0	-	Antitoxin
-	59206..59268	-	63	NuclAT_0	-	Antitoxin
-	59206..59268	-	63	NuclAT_0	-	Antitoxin
FQU84_RS01185	59318..59470	+	153	WP_024179514.1	Hok/Gef family protein	Toxin
FQU84_RS01190	59542..59793	-	252	WP_001291965.1	hypothetical protein	-
-	60186..60237	-	52	NuclAT_1	-	-
-	60186..60237	-	52	NuclAT_1	-	-
-	60186..60237	-	52	NuclAT_1	-	-
-	60186..60237	-	52	NuclAT_1	-	-
FQU84_RS27055	61077..61253	-	177	WP_001054898.1	hypothetical protein	-
FQU84_RS01200	61462..61671	-	210	WP_015059315.1	hemolysin expression modulator Hha	-
FQU84_RS01205	61769..62383	-	615	WP_015059316.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..102714	102714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5795.12 Da
• Isoelectric Point: 8.7948

>T135077 WP_024179514.1 NZ_CP042248:59318-59470 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T135077 NZ_CP042248:59318-59470 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGATTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT135077 NZ_CP042248:c59268-59206 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin