Detailed information of TA system TA135068

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67902..68156
• Replicon: plasmid pBCE049-1
• Accession: NZ_CP042247
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FQU84_RS00340
• Protein ID: WP_047649939.1
• Coordinates: 67902..68108 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	68095..68156 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS00300	63105..64109	-	1005	WP_050939068.1	IS110 family transposase	-
FQU84_RS00305	64179..65075	+	897	Protein_53	IS3 family transposase	-
FQU84_RS27030	65108..65284	-	177	WP_157905840.1	hypothetical protein	-
FQU84_RS00320	66199..67056	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
FQU84_RS00325	67049..67123	-	75	WP_001365705.1	RepA leader peptide Tap	-
FQU84_RS00335	67370..67618	-	249	WP_000083838.1	replication regulatory protein RepA	-
FQU84_RS00340	67902..68108	-	207	WP_047649939.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	68095..68156	+	62	NuclAT_1	-	Antitoxin
-	68095..68156	+	62	NuclAT_1	-	Antitoxin
-	68095..68156	+	62	NuclAT_1	-	Antitoxin
-	68095..68156	+	62	NuclAT_1	-	Antitoxin
FQU84_RS00350	68331..68713	-	383	Protein_59	hypothetical protein	-
FQU84_RS00355	68816..69277	-	462	WP_047649930.1	thermonuclease family protein	-
FQU84_RS00365	69974..70177	-	204	WP_001327131.1	hypothetical protein	-
FQU84_RS00370	70332..70889	-	558	WP_047649932.1	fertility inhibition protein FinO	-
FQU84_RS00375	70992..71852	-	861	WP_000704512.1	alpha/beta hydrolase	-
FQU84_RS00380	71911..72657	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cdtC / cdtB / cdtA / cnf1 / hlyD / hlyB / hlyA / hlyC / iutA / iucD / iucC / iucB / iucA	1..142705	142705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7762.27 Da
• Isoelectric Point: 8.8807

>T135068 WP_047649939.1 NZ_CP042247:c68108-67902 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGGLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T135068 NZ_CP042247:c68108-67902 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGGGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT135068 NZ_CP042247:68095-68156 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin