Detailed information of TA system TA135060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3941241..3941935
• Replicon: chromosome
• Accession: NZ_CP042246
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: FQU83_RS21445
• Protein ID: WP_001263500.1
• Coordinates: 3941241..3941639 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FQU83_RS21450
• Protein ID: WP_000554758.1
• Coordinates: 3941642..3941935 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS21420	3936606..3937064	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
FQU83_RS21425	3937325..3938782	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
FQU83_RS21430	3938839..3939453	-	615	WP_000602123.1	peptide chain release factor H	-
FQU83_RS21435	3939450..3940589	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
FQU83_RS21440	3940779..3941231	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
FQU83_RS21445	3941241..3941639	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FQU83_RS21450	3941642..3941935	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FQU83_RS21455	3941987..3943042	-	1056	WP_001226168.1	DNA polymerase IV	-
FQU83_RS21460	3943113..3943898	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
FQU83_RS21465	3943870..3945582	+	1713	Protein_3810	flagellar biosynthesis protein FlhA	-
FQU83_RS21470	3945661..3945819	+	159	WP_014639450.1	hypothetical protein	-
FQU83_RS21475	3945902..3946399	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3941241..3961595	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T135060 WP_001263500.1 NZ_CP042246:c3941639-3941241 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T135060 NZ_CP042246:c3941639-3941241 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT135060 WP_000554758.1 NZ_CP042246:c3941935-3941642 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT135060 NZ_CP042246:c3941935-3941642 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3