Detailed information of TA system TA135029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102008..102433
• Replicon: plasmid pColV-PU1
• Accession: NZ_CP042245
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: FQU83_RS00705
• Protein ID: WP_001332444.1
• Coordinates: 102008..102169 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	102222..102433 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS00670	97358..97585	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
FQU83_RS00675	97679..98365	-	687	WP_000332490.1	PAS domain-containing protein	-
FQU83_RS00680	98555..98938	-	384	WP_000124981.1	relaxosome protein TraM	-
FQU83_RS00690	99272..99862	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
FQU83_RS00695	100158..100979	-	822	WP_001234485.1	DUF945 domain-containing protein	-
FQU83_RS00700	101158..101770	+	613	Protein_121	hypothetical protein	-
FQU83_RS00705	102008..102169	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	102222..102433	-	212	NuclAT_1	-	Antitoxin
-	102222..102433	-	212	NuclAT_1	-	Antitoxin
-	102222..102433	-	212	NuclAT_1	-	Antitoxin
-	102222..102433	-	212	NuclAT_1	-	Antitoxin
FQU83_RS00715	102444..103163	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
FQU83_RS00720	103160..103594	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
FQU83_RS00725	103649..105607	-	1959	Protein_125	ParB/RepB/Spo0J family partition protein	-
FQU83_RS00730	105666..105899	-	234	WP_001209608.1	DUF905 domain-containing protein	-
FQU83_RS00735	105962..106459	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..285922	285922
	-	flank	IS/Tn	-	-	107305..107808	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T135029 WP_001332444.1 NZ_CP042245:c102169-102008 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T135029 NZ_CP042245:c102169-102008 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT135029 NZ_CP042245:c102433-102222 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin