Detailed information of TA system TA135023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17573..17999
• Replicon: plasmid pColV-PU1
• Accession: NZ_CP042245
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FQU83_RS00135
• Protein ID: WP_001312861.1
• Coordinates: 17573..17731 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	17775..17999 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS00090	12633..12860	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FQU83_RS00095	12954..13640	-	687	WP_000332484.1	PAS domain-containing protein	-
FQU83_RS00100	13831..14214	-	384	WP_000124981.1	relaxosome protein TraM	-
FQU83_RS00105	14480..15127	+	648	WP_000614934.1	transglycosylase SLT domain-containing protein	-
FQU83_RS00110	15424..16245	-	822	WP_145607291.1	DUF945 domain-containing protein	-
FQU83_RS00115	16364..16651	-	288	WP_101357019.1	hypothetical protein	-
FQU83_RS27190	17007..17219	+	213	WP_162491396.1	hypothetical protein	-
FQU83_RS00135	17573..17731	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	17775..17999	-	225	NuclAT_0	-	Antitoxin
-	17775..17999	-	225	NuclAT_0	-	Antitoxin
-	17775..17999	-	225	NuclAT_0	-	Antitoxin
-	17775..17999	-	225	NuclAT_0	-	Antitoxin
FQU83_RS00140	17811..17990	+	180	WP_001334662.1	hypothetical protein	-
FQU83_RS00145	18011..18730	-	720	WP_021573167.1	plasmid SOS inhibition protein A	-
FQU83_RS00150	18727..19161	-	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
FQU83_RS00155	19216..21174	-	1959	WP_101357017.1	ParB/RepB/Spo0J family partition protein	-
FQU83_RS00160	21239..21472	-	234	WP_000006030.1	DUF905 family protein	-
FQU83_RS00165	21528..22067	-	540	WP_009426937.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..285922	285922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T135023 WP_001312861.1 NZ_CP042245:c17731-17573 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T135023 NZ_CP042245:c17731-17573 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT135023 NZ_CP042245:c17999-17775 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin