Detailed information of TA system TA134973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 5224841..5225631
• Replicon: chromosome
• Accession: NZ_CP042180
• Organism: Pseudomonas sp. KBS0802

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q88NE5
• Locus tag: FFH79_RS24715
• Protein ID: WP_010952397.1
• Coordinates: 5225164..5225631 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: I7C311
• Locus tag: FFH79_RS24710
• Protein ID: WP_014859917.1
• Coordinates: 5224841..5225167 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFH79_RS24685	5220375..5221436	-	1062	WP_049587435.1	HlyD family secretion protein	-
FFH79_RS24690	5221465..5223006	-	1542	WP_010952401.1	MFS transporter	-
FFH79_RS24695	5223123..5224028	-	906	WP_010952400.1	LysR family transcriptional regulator	-
FFH79_RS24705	5224302..5224748	+	447	WP_010952399.1	universal stress protein	-
FFH79_RS24710	5224841..5225167	+	327	WP_014859917.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
FFH79_RS24715	5225164..5225631	+	468	WP_010952397.1	type II toxin-antitoxin system YhaV family toxin	Toxin
FFH79_RS24720	5225704..5226564	-	861	WP_010952396.1	HlyD family secretion protein	-
FFH79_RS24725	5226575..5226775	-	201	WP_003251718.1	DUF1656 domain-containing protein	-
FFH79_RS24730	5226765..5228942	-	2178	WP_003251716.1	FUSC family protein	-
FFH79_RS24735	5228939..5230468	-	1530	WP_010952395.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (17-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17962.34 Da
• Isoelectric Point: 9.7963

>T134973 WP_010952397.1 NZ_CP042180:5225164-5225631 [Pseudomonas sp. KBS0802]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Nucleotide

• Length: 468 bp

>T134973 NZ_CP042180:5225164-5225631 [Pseudomonas sp. KBS0802]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGATCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCAACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11919.40 Da
• Isoelectric Point: 4.3722

>AT134973 WP_014859917.1 NZ_CP042180:5224841-5225167 [Pseudomonas sp. KBS0802]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Nucleotide

• Length: 327 bp

>AT134973 NZ_CP042180:5224841-5225167 [Pseudomonas sp. KBS0802]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Structures

Toxin

• AlphaFold DB: Q88NE5

Antitoxin

• AlphaFold DB: I7C311