Detailed information of TA system TA134952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5553217..5553830
• Replicon: chromosome
• Accession: NZ_CP042176
• Organism: Bradyrhizobium sp. KBS0727

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FFI89_RS26065
• Protein ID: WP_138830419.1
• Coordinates: 5553217..5553513 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFI89_RS26070
• Protein ID: WP_138830420.1
• Coordinates: 5553522..5553830 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFI89_RS26055	5549215..5551656	-	2442	WP_138830417.1	hypothetical protein	-
FFI89_RS26060	5551796..5552947	-	1152	WP_138830418.1	putative lipid II flippase FtsW	-
FFI89_RS26065	5553217..5553513	+	297	WP_138830419.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFI89_RS26070	5553522..5553830	+	309	WP_138830420.1	HigA family addiction module antidote protein	Antitoxin
FFI89_RS26075	5554070..5555380	+	1311	WP_138830421.1	ABC transporter substrate-binding protein	-
FFI89_RS26080	5555484..5556653	-	1170	WP_138830422.1	Gfo/Idh/MocA family oxidoreductase	-
FFI89_RS26085	5556715..5558115	-	1401	WP_138830423.1	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11459.93 Da
• Isoelectric Point: 6.7095

>T134952 WP_138830419.1 NZ_CP042176:5553217-5553513 [Bradyrhizobium sp. KBS0727]
MIVGFRDGWLRAFFVEDTKSRKIPADLENRLFRKIQMIDDAATDQDLRVPPSNHFEKLRGNLDGFHSIRVNQQCRLIFQW
DGSCGEATGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T134952 NZ_CP042176:5553217-5553513 [Bradyrhizobium sp. KBS0727]
ATGATTGTTGGCTTTCGAGATGGCTGGCTGCGCGCCTTCTTCGTCGAGGATACAAAATCCAGGAAAATTCCTGCTGATCT
GGAAAATCGCTTGTTTCGAAAAATCCAGATGATCGACGATGCCGCGACGGATCAGGACTTGCGGGTGCCACCTAGCAATC
ATTTCGAAAAGCTACGCGGCAATCTGGATGGATTCCACTCCATTCGCGTTAATCAGCAATGTCGGTTGATTTTCCAGTGG
GACGGCAGCTGTGGTGAAGCGACCGGCATTTATCTGGACGATCATTCCTATCGGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11572.48 Da
• Isoelectric Point: 11.2338

>AT134952 WP_138830420.1 NZ_CP042176:5553522-5553830 [Bradyrhizobium sp. KBS0727]
MLTTKRKPASVGEILLEEFMEPMGLTQGALAKAMGVQRKHVNELCNDRRTVTASTALILARVFGNSPDFWLNVQRRGDLW
DAMHSPRERERIKRARPLKSVA

Nucleotide

• Length: 309 bp

>AT134952 NZ_CP042176:5553522-5553830 [Bradyrhizobium sp. KBS0727]
ATGTTGACGACGAAGCGCAAGCCGGCGAGCGTCGGTGAAATTCTGCTTGAAGAGTTCATGGAACCGATGGGTTTGACCCA
AGGCGCCCTTGCCAAGGCCATGGGGGTACAGCGCAAGCACGTAAACGAGCTGTGCAACGATCGTCGCACCGTCACCGCGT
CCACGGCGCTGATCCTTGCCCGCGTGTTCGGCAATAGCCCGGATTTCTGGCTGAACGTGCAGCGGCGCGGCGATTTGTGG
GATGCGATGCATTCGCCCCGCGAACGCGAGCGGATCAAACGCGCTCGGCCACTGAAATCGGTCGCATGA