Detailed information of TA system TA134949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5553179..5553792
• Replicon: chromosome
• Accession: NZ_CP042175
• Organism: Bradyrhizobium sp. KBS0725

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FFI71_RS26070
• Protein ID: WP_138830419.1
• Coordinates: 5553179..5553475 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFI71_RS26075
• Protein ID: WP_138830420.1
• Coordinates: 5553484..5553792 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFI71_RS26060	5549177..5551618	-	2442	WP_138830417.1	hypothetical protein	-
FFI71_RS26065	5551758..5552909	-	1152	WP_138830418.1	putative lipid II flippase FtsW	-
FFI71_RS26070	5553179..5553475	+	297	WP_138830419.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFI71_RS26075	5553484..5553792	+	309	WP_138830420.1	HigA family addiction module antidote protein	Antitoxin
FFI71_RS26080	5554032..5555342	+	1311	WP_138830421.1	ABC transporter substrate-binding protein	-
FFI71_RS26085	5555446..5556615	-	1170	WP_138830422.1	Gfo/Idh/MocA family oxidoreductase	-
FFI71_RS26090	5556677..5558077	-	1401	WP_138830423.1	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11459.93 Da
• Isoelectric Point: 6.7095

>T134949 WP_138830419.1 NZ_CP042175:5553179-5553475 [Bradyrhizobium sp. KBS0725]
MIVGFRDGWLRAFFVEDTKSRKIPADLENRLFRKIQMIDDAATDQDLRVPPSNHFEKLRGNLDGFHSIRVNQQCRLIFQW
DGSCGEATGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T134949 NZ_CP042175:5553179-5553475 [Bradyrhizobium sp. KBS0725]
ATGATTGTTGGCTTTCGAGATGGCTGGCTGCGCGCCTTCTTCGTCGAGGATACAAAATCCAGGAAAATTCCTGCTGATCT
GGAAAATCGCTTGTTTCGAAAAATCCAGATGATCGACGATGCCGCGACGGATCAGGACTTGCGGGTGCCACCTAGCAATC
ATTTCGAAAAGCTACGCGGCAATCTGGATGGATTCCACTCCATTCGCGTTAATCAGCAATGTCGGTTGATTTTCCAGTGG
GACGGCAGCTGTGGTGAAGCGACCGGCATTTATCTGGACGATCATTCCTATCGGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11572.48 Da
• Isoelectric Point: 11.2338

>AT134949 WP_138830420.1 NZ_CP042175:5553484-5553792 [Bradyrhizobium sp. KBS0725]
MLTTKRKPASVGEILLEEFMEPMGLTQGALAKAMGVQRKHVNELCNDRRTVTASTALILARVFGNSPDFWLNVQRRGDLW
DAMHSPRERERIKRARPLKSVA

Nucleotide

• Length: 309 bp

>AT134949 NZ_CP042175:5553484-5553792 [Bradyrhizobium sp. KBS0725]
ATGTTGACGACGAAGCGCAAGCCGGCGAGCGTCGGTGAAATTCTGCTTGAAGAGTTCATGGAACCGATGGGTTTGACCCA
AGGCGCCCTTGCCAAGGCCATGGGGGTACAGCGCAAGCACGTAAACGAGCTGTGCAACGATCGTCGCACCGTCACCGCGT
CCACGGCGCTGATCCTTGCCCGCGTGTTCGGCAATAGCCCGGATTTCTGGCTGAACGTGCAGCGGCGCGGCGATTTGTGG
GATGCGATGCATTCGCCCCGCGAACGCGAGCGGATCAAACGCGCTCGGCCACTGAAATCGGTCGCATGA