Detailed information of TA system TA134814

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 230462..230888
• Replicon: plasmid pAR349
• Accession: NZ_CP041997
• Organism: Escherichia coli strain AR Bank #0349

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FN958_RS25505
• Protein ID: WP_001312861.1
• Coordinates: 230462..230620 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	230664..230888 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FN958_RS25465	225834..226523	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
FN958_RS25470	226710..227093	-	384	WP_001151524.1	relaxosome protein TraM	-
FN958_RS25475	227414..228016	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
FN958_RS25480	228313..229134	-	822	WP_001234469.1	DUF945 domain-containing protein	-
FN958_RS25485	229253..229540	-	288	WP_000107535.1	hypothetical protein	-
FN958_RS25490	229565..229771	-	207	WP_000547971.1	hypothetical protein	-
FN958_RS25505	230462..230620	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	230664..230888	-	225	NuclAT_0	-	Antitoxin
-	230664..230888	-	225	NuclAT_0	-	Antitoxin
-	230664..230888	-	225	NuclAT_0	-	Antitoxin
-	230664..230888	-	225	NuclAT_0	-	Antitoxin
FN958_RS25985	230700..230888	+	189	WP_001299721.1	hypothetical protein	-
FN958_RS25510	230900..231619	-	720	WP_000116352.1	plasmid SOS inhibition protein A	-
FN958_RS25515	231616..232050	-	435	WP_064770609.1	conjugation system SOS inhibitor PsiB	-
FN958_RS25520	232105..232302	-	198	Protein_251	hypothetical protein	-
FN958_RS25525	232330..232563	-	234	WP_000005987.1	DUF905 family protein	-
FN958_RS25530	232631..233170	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
FN958_RS25535	233196..233402	-	207	WP_000275856.1	hypothetical protein	-
FN958_RS25555	234541..235512	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / mcr-1.1 / mph(A) / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / floR / sitABCD	iutA / iucD / iucC / iucB / iucA	1..278067	278067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T134814 WP_001312861.1 NZ_CP041997:c230620-230462 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T134814 NZ_CP041997:c230620-230462 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT134814 NZ_CP041997:c230888-230664 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin