Detailed information of TA system TA134809

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 192131..192385
• Replicon: plasmid pAR349
• Accession: NZ_CP041997
• Organism: Escherichia coli strain AR Bank #0349

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FN958_RS25265
• Protein ID: WP_001351576.1
• Coordinates: 192131..192337 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	192324..192385 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FN958_RS25220	187683..188084	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FN958_RS25225	188017..188274	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FN958_RS25230	188367..189020	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FN958_RS25240	189959..190816	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
FN958_RS25245	190809..191291	-	483	WP_001273588.1	hypothetical protein	-
FN958_RS25250	191284..191358	-	75	WP_001442103.1	RepA leader peptide Tap	-
FN958_RS25260	191590..191847	-	258	WP_000083845.1	replication regulatory protein RepA	-
FN958_RS25265	192131..192337	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	192324..192385	+	62	NuclAT_1	-	Antitoxin
-	192324..192385	+	62	NuclAT_1	-	Antitoxin
-	192324..192385	+	62	NuclAT_1	-	Antitoxin
-	192324..192385	+	62	NuclAT_1	-	Antitoxin
FN958_RS25980	192641..192715	-	75	Protein_203	endonuclease	-
FN958_RS25275	192961..193173	-	213	WP_001299730.1	ANR family transcriptional regulator	-
FN958_RS25280	193309..193869	-	561	WP_000139315.1	fertility inhibition protein FinO	-
FN958_RS25285	193972..194832	-	861	WP_000704513.1	alpha/beta hydrolase	-
FN958_RS25290	194891..195637	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / mcr-1.1 / mph(A) / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / floR / sitABCD	iutA / iucD / iucC / iucB / iucA	1..278067	278067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T134809 WP_001351576.1 NZ_CP041997:c192337-192131 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T134809 NZ_CP041997:c192337-192131 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT134809 NZ_CP041997:192324-192385 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin