Detailed information of TA system TA134747

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43027..43296
• Replicon: plasmid pEC2_2
• Accession: NZ_CP041957
• Organism: Escherichia coli strain EC2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FOZ67_RS23845
• Protein ID: WP_001312861.1
• Coordinates: 43138..43296 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	43027..43092 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ67_RS23815	38737..39264	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FOZ67_RS23820	39322..39555	+	234	WP_000006003.1	DUF905 family protein	-
FOZ67_RS23825	39616..41639	+	2024	Protein_48	ParB/RepB/Spo0J family partition protein	-
FOZ67_RS23830	41708..42142	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FOZ67_RS23835	42139..42858	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	42870..43094	+	225	NuclAT_0	-	-
-	42870..43094	+	225	NuclAT_0	-	-
-	42870..43094	+	225	NuclAT_0	-	-
-	42870..43094	+	225	NuclAT_0	-	-
FOZ67_RS23840	42879..43058	-	180	WP_001309233.1	hypothetical protein	-
-	43027..43092	+	66	-	-	Antitoxin
FOZ67_RS23845	43138..43296	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FOZ67_RS25270	43534..43911	-	378	Protein_53	hypothetical protein	-
FOZ67_RS23855	44211..44507	+	297	WP_001272251.1	hypothetical protein	-
FOZ67_RS23860	44618..45439	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FOZ67_RS23865	45736..46338	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FOZ67_RS23870	46661..47044	+	384	WP_001354030.1	relaxosome protein TraM	-
FOZ67_RS23875	47238..47909	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FOZ67_RS23880	48046..48273	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..83648	83648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T134747 WP_001312861.1 NZ_CP041957:43138-43296 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T134747 NZ_CP041957:43138-43296 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT134747 NZ_CP041957:43027-43092 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin