Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1858202..1858704 Replicon chromosome
Accession NZ_CP041955
Organism Escherichia coli strain EC2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FOZ67_RS08790 Protein ID WP_000767829.1
Coordinates 1858202..1858456 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FOZ67_RS08795 Protein ID WP_001259255.1
Coordinates 1858453..1858704 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOZ67_RS08760 1853217..1853444 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FOZ67_RS08765 1853458..1854516 - 1059 WP_000492321.1 transport protein YeeE -
FOZ67_RS08770 1854695..1856053 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FOZ67_RS08775 1856043..1856105 - 63 WP_010723108.1 membrane protein YoeI -
FOZ67_RS08780 1856320..1857249 - 930 WP_000803351.1 LysR family transcriptional regulator -
FOZ67_RS08785 1857295..1858119 - 825 WP_000754749.1 SDR family oxidoreductase -
FOZ67_RS08790 1858202..1858456 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FOZ67_RS08795 1858453..1858704 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FOZ67_RS08800 1858988..1859038 + 51 WP_001364200.1 his operon leader peptide -
FOZ67_RS08805 1859184..1860083 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
FOZ67_RS08810 1860089..1861393 + 1305 WP_001446927.1 histidinol dehydrogenase -
FOZ67_RS08815 1861390..1862460 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
FOZ67_RS08820 1862460..1863527 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T134728 WP_000767829.1 NZ_CP041955:c1858456-1858202 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T134728 NZ_CP041955:c1858456-1858202 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT134728 WP_001259255.1 NZ_CP041955:c1858704-1858453 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT134728 NZ_CP041955:c1858704-1858453 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References