Detailed information of TA system TA134716

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34685..34955
• Replicon: plasmid pKP2_2
• Accession: NZ_CP041948
• Organism: Klebsiella pneumoniae strain KP2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FOZ68_RS26615
• Protein ID: WP_001312861.1
• Coordinates: 34797..34955 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34685..34748 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ68_RS26590	30396..30923	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FOZ68_RS26595	30981..31214	+	234	WP_000006003.1	DUF905 family protein	-
FOZ68_RS26600	31275..33298	+	2024	Protein_39	ParB/RepB/Spo0J family partition protein	-
FOZ68_RS26605	33367..33801	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FOZ68_RS26610	33798..34517	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34529..34753	+	225	NuclAT_0	-	-
-	34529..34753	+	225	NuclAT_0	-	-
-	34529..34753	+	225	NuclAT_0	-	-
-	34529..34753	+	225	NuclAT_0	-	-
-	34685..34748	-	64	-	-	Antitoxin
FOZ68_RS26615	34797..34955	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FOZ68_RS28835	35193..35570	-	378	Protein_43	hypothetical protein	-
FOZ68_RS26625	35870..36166	+	297	WP_001272251.1	hypothetical protein	-
FOZ68_RS26630	36277..37098	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FOZ68_RS26635	37395..37997	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FOZ68_RS26640	38320..38703	+	384	WP_001354030.1	relaxosome protein TraM	-
FOZ68_RS26645	38897..39568	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FOZ68_RS26650	39705..39932	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / dfrA12	-	1..75307	75307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T134716 WP_001312861.1 NZ_CP041948:34797-34955 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T134716 NZ_CP041948:34797-34955 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT134716 NZ_CP041948:c34748-34685 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin