Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 11887..12451 Replicon chromosome
Accession NZ_CP041944
Organism Lacticaseibacillus paracasei strain NJ

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag FO269_RS00060 Protein ID WP_016363448.1
Coordinates 11887..12189 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag FO269_RS00065 Protein ID WP_003570051.1
Coordinates 12182..12451 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FO269_RS00040 7200..7757 + 558 WP_003584278.1 helix-turn-helix domain-containing protein -
FO269_RS00045 8182..10359 - 2178 WP_003570057.1 RNA-binding transcriptional accessory protein -
FO269_RS00050 10595..11287 - 693 WP_003570055.1 hypothetical protein -
FO269_RS15835 11405..11548 - 144 WP_162259784.1 hypothetical protein -
FO269_RS00060 11887..12189 - 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
FO269_RS00065 12182..12451 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
FO269_RS00070 12825..13478 - 654 WP_003570049.1 GNAT family N-acetyltransferase -
FO269_RS00075 13523..14152 - 630 WP_144182028.1 DUF2399 domain-containing protein -
FO269_RS00080 14215..14541 - 327 WP_003584272.1 YrdB family protein -
FO269_RS00085 14534..14938 - 405 WP_003570045.1 MerR family transcriptional regulator -
FO269_RS00090 15136..16362 + 1227 WP_003570043.1 hypothetical protein -
FO269_RS00095 16559..17446 + 888 WP_144182030.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T134691 WP_016363448.1 NZ_CP041944:c12189-11887 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T134691 NZ_CP041944:c12189-11887 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT134691 WP_003570051.1 NZ_CP041944:c12451-12182 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT134691 NZ_CP041944:c12451-12182 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References