Detailed information of TA system TA134643

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84718..85144
• Replicon: plasmid unnamed1
• Accession: NZ_CP041920
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FPV29_RS23510
• Protein ID: WP_001312861.1
• Coordinates: 84718..84876 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84920..85144 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS23485	79872..82499	-	2628	WP_001064245.1	type IV secretion system protein TraC	-
FPV29_RS23490	82697..83394	+	698	Protein_88	IS1-like element IS1A family transposase	-
FPV29_RS23495	83510..83797	-	288	WP_000107535.1	hypothetical protein	-
FPV29_RS23510	84718..84876	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84920..85144	-	225	NuclAT_0	-	Antitoxin
-	84920..85144	-	225	NuclAT_0	-	Antitoxin
-	84920..85144	-	225	NuclAT_0	-	Antitoxin
-	84920..85144	-	225	NuclAT_0	-	Antitoxin
FPV29_RS24945	84956..85144	+	189	WP_001299721.1	hypothetical protein	-
FPV29_RS23515	85156..85875	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
FPV29_RS23520	85872..86306	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
FPV29_RS23525	86361..86558	-	198	Protein_94	hypothetical protein	-
FPV29_RS23530	86586..86819	-	234	WP_000005987.1	DUF905 family protein	-
FPV29_RS23535	86887..87426	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
FPV29_RS23540	87452..87658	-	207	WP_000275856.1	hypothetical protein	-
FPV29_RS23560	88797..89768	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aadA5 / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..122471	122471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T134643 WP_001312861.1 NZ_CP041920:c84876-84718 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T134643 NZ_CP041920:c84876-84718 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT134643 NZ_CP041920:c85144-84920 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin